DESCRIPTION: Homocitrate synthase is involved in lysine biosynthesis in yeast. The genome of Saccharomyces cerevisiae encodes two homocitrate synthase isozymes, Lys20p and Lys21p, of molecular weights 47.1 and 48.6 kDa, respectively. Both proteins are found within the nucleus of the cell. The two proteins are ~90% conserved and this antibody is capable of recognizing both isozymes
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SPECIES CROSS-REACTIVITY:
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Yst
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APPLICATIONS/DILUTIONS: |
ICC/IF (1/500 – 1/2,500)
WB (1/500 – 1/10,000)
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SOURCE: |
Mice were immunized with yeast nuclear protein preparations and hybridomas were screened against yeast cells (IF) and yeast protein homogenates (WB).
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FORM/STORAGE: |
0.2 ml (filtered ascites) with 50% glycerol, sodium azide and 1.0 mg/ml BSA. Store at -20º C. Avoid multiple freeze/thaw cycles. Immunoglobulin isotype is unknown.
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THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR DIAGNOSTIC PROCEDURES.
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