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Neurofilament, NF-H (Clone 9B12)

Mouse Monoclonal Antibody

PRODUCT #: 40-1276

 

 

DESCRIPTION: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. This antibody reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites.
   
SPECIES CROSS-REACTIVITY: Bovine, human, mouse, rat

 

APPLICATIONS/DILUTIONS:        ICC/IF (1/200)
WB (1/2,000)
   
SOURCE: Mice were immunized with purified bovine spinal cord NF-H.
   
FORM/STORAGE: 0.1 ml with 50% glycerol, 0.01% sodium azide. 
Store at -20º C.  Avoid multiple freeze/thaw cycles.  Immunoglobulin isotype is IgG2b.
   
Reference: Harris, J., Ayyub, C. and Shaw G. J Neurosci Res 30:47-62 1991.
   
 
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR DIAGNOSTIC PROCEDURES.