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Ubiquitin-1 (Clone UBI-1 AP)

Mouse Monoclonal Antibody

PRODUCT #: 40-1287

 

 

DESCRIPTION: Ubiquitin-1 is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has an important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalantly coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubquitinated complex is then recognized by a complex of degradative enzymes which together form the proteosome. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions seen in serious human disease states which appear to be resistant to normal degradation, so that ubiquitin antibodies are very useful for studies of these inclusions. For example the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, the Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can all be readily visualized with ubiquitin antibodies of appropriate specificity.
   
SPECIES CROSS-REACTIVITY: Broad

 

APPLICATIONS/DILUTIONS:        ICC/IF (1/500)
WB (1/1,000)
   
SOURCE: Mice were immunized with purified human ubiquitin.
   
FORM/ STORAGE: 0.1 ml of protein G purifies IgG with 50% glycerol, 0.01% sodium azide. Store at -20º C. Avoid multiple freeze/thaw cycles.  Immunoglobulin isotype is IgG1.
   
Reference: Perry, G. et al. Proc. Natl. Acad. Sci. USA 84, 3033-3036 (1987)
Shaw, G. and Chau, V. Proc. Natl. Acad. Sci. USA 85, 2854-2858 (1988)
Hirano, S., et al. Cell 70: 293-301 (1992)
Cuervo, A.M., et al. Mol. Biol. 9: 1995-2010 (1995)
Sternsdorf, T., et al. J. Cell Biol. 139: 1621-1634 (1997)
Tae-Wan Kim, et al. J. Biol. Chem. 272: 11006-11010 (1997)
Verdier, F., et al. J. Cell Biol. 273: 28185-28190 (1998)
Laroia, G., et al. Science 284: 499-502 (1999)
   
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR DIAGNOSTIC PROCEDURES.