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Ubiquitin C-Terminal Hydrolase 1 (Clone BH7)

Mouse Monoclonal Antibody

PRODUCT #: 40-1288

 

 

DESCRIPTION: Ubiquitin C-terminal hydroalse 1 (UBCHL1) has several other names, such as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. For example the Park5 gene causes one form of human Parkinson's disease, and proves to be a point mutation in the UCHL1 gene producing a I93M form of the UCHL1 protein which has reduced ubiquitin hydrolase activity. Interestingly a common allelic variant of UCHL1, the S18Y polymorphism is actually protective against Parkinson's disease. Recent studies suggest that UCHL1 also has a ubiqutinyl ligase activity, being able to couple ubiquitin monomers by linking the C-terminus of one with lysine 63 of the other. Since UCHL1 is heavily expressed in neurons, antibodies to UCHL1 can be used to identify neurons in histological sections and in tissue culture. The great abundance of this protein in neurons means that it is released from neurons in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker of neuronal injury or degeneration.
   
SPECIES CROSS-REACTIVITY: Bovine, human, mouse, rat

 

APPLICATIONS/DILUTIONS:        ICC/IF (1/200)
WB (1/2,000)
   
SOURCE: Mice were immunized with recombinant full length human UCHL1 purified from E. coli.
   
FORM/ STORAGE: 0.1 ml with 50% glycerol, 0.01% sodium azide. Store at -20º C.  Avoid multiple freeze/thaw cycles. Immunoglobulin isotype is IgG1.
   
Reference: Liu Y, et al. Cell 111:209-18 (2002).
   
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR DIAGNOSTIC PROCEDURES.