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p130/Rb2 (Clone DCS211.6)

Mouse Monoclonal Antibody

PRODUCT #: 45-6796

 

DESCRIPTION: p130/pRb2, p107 and Rb together make up the Rb family of cell cycle regulatory proteins.  As a family of proteins, the different members share several structural and functional features which include two distinct "pocket domains", interaction with viral gene products, interaction with members of the E2F family of proteins and changes in phosphorylation states leading to modulation of activity and cell cycle expression.  The p130 protein is ubiquitously expressed with highest levels in terminally differentiated cells where it presumably functions to maintain the cells in a G0 state and as such has been used as a marker for G0.  Depending on position within the cell cycle, p130 can exist in 4 different forms as a function of phosphorylation with Form 0 being the unphosphorylated form and the other three forms being referred to as Forms 1-3 correlating with increasing degrees of phosphorylation.  Changes in levels of p130 expression have been reported in several cancers including endometrial, small cell lung, and neuroblastoma.


SPECIES CROSS-REACTIVITY:


Human, mous


APPLICATIONS/DILUTIONS:


Western blot (1.0 ug/ml 


SOURCE:


Balb/c mice were immunized with human p130 protein and fusing the splenocytes with Sp2/0 mouse myeloma cells. 


FORM/STORAGE:


100 ug purified IgG1 with 50% glycerol, 0.01% sodium azide and 1.0 mg/ml BSA. Store at -20º C. Avoid multiple freeze/thaw cycles.


THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR DIAGNOSTIC PROCEDURES.